DAPI (2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride) staining solution is a kind of fluorescent dye that luoresces brightly upon selectively binding to the minor groove of double stranded DNA. It is essentially excluded from viable cells, but can penetrate cell membranes of dead or dying cells. The fluorescence of this blue fluorescent probe is approximately 20-fold greater than in the nonbound state. Therefore, DAPI is used for evaluation of cell death and apoptosis of unfixed cells in flow cytometry. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
Features:
1. Convenient—Ready-to-use liquid (10 ug/mL)
2. Wide application—Can be used with live cells and fixed cells.
3. Cellular imaging—Cell-permeable dye is effective for fixed-cell staining and quantitation of DNA content
Component:10 ug/mL
Storage: -20℃ Protect from light
Shelf Life:Store at -20℃ Protect from light for 1 year.
Item No | BL105A |
Size | 10ml |
Brand | Biosharp |
Manual Download | Download |