Item No:BL711A
Size:100T
Brand:biosharp
15 PVQuantity:
C-1 Mitochondrial Membrane Potential Detection Kit provides a quick and reliable method to detect the mitochondrial membrane potential changes in apoptotic cells using flow cytometry. The loss of mitochondrial membrane potential is a hallmark for apoptosis.
Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. In healthy cells, the JC-1 accumulates in the mitochondrial to form J-aggregates, which become red fluorescence. In apoptotic cells, the JC-1 cannot accumulate within the mitochondria and remains in the cytoplasm in monomeric form to show green fluorescence.
Features:
1. Simple workflow—add, incubate, and read format, no wash needed
2. Flexibility—suitable for flow cytometry, fluorescence microscope, and plate reader
3. Fast—results in as little as 30 minutes
Component:1. JC-1(200×) 100μL×5
2. CCCP(10mM) 20μL
3. Ultrapure Water 90mL
4. JC-1 Stain Buffer(5×) 80mL
Storage: -20℃. Protect from light.
Shelf Life:Store at -20℃ for 1 year.
Item No | BL711A |
Size | 100T |
Brand | biosharp |
Manual Download | Download |