The Western Blot Stripping Buffer is a solution for removing primary and secondary antibodies from probed Western blot membranes. Stripping refers to the method of removing a primary and secondary antibody from a western blot membrane. A single blot can be analyzed sequentially with multiple antibodies by stripping one antibody from the blot and subsequently incubating with an additional antibody.
This product is composed of Tris-Hcl buffer, SDS and β-mercaptoethanol. Antibody removal with this buffer can occur under mild conditions (room temperature, 30 min incubation), minimizing loss of immobilized protein from the membrane.
Features:
1. Convenient—Ready to use
2. Efficient—Strip blots in only 10-20 minutes at room temperature
3. Save time—No need to run new gels and prepare a new blot
4. Low requirements—Incubation at room temperature
Component:N/A
Storage: 4°C
Shelf Life:Stored at 4°C for 6 months.
Item No | BL521A |
Size | 500T |
Brand | Biosharp |
Manual Download | Download |