IPTG (isopropyl-beta-D-thiogalactopyranoside) is an analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization.
This product is a 50 mg/ml solution by dissolving 250mg of IPTG in 5 ml of distilled H2O. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.
2. Physical State—Liquid
Shelf Life： Store at -20°C for 1 year