The compound 5-bromo4-chloro-3-indolyl β-D-glucuronide (X-Gluc) is a GUS substrate, which upon hydrolysis produces an indigoblue precipitate. It is used in histochemical staining of plant tissues expressing the E. coli GUS enzyme. X-Gluc is applied to tissue sections in phosphate buffer, pH 7.0, containing potassium ferricyanide and potassium ferrocyanide. The substrate penetrates the tissue and is hydrolyzed by the β-glucuronidase. The indolyl moiety obtained is then oxidized to an indoxyl radical in a reaction catalyzed by the ferricyanide/ferrocyanide mixture. The indoxyl moiety dimerizes to form an insoluble indigo-blue derivative.

Features:

1. Detection of GUS expression in plant cells and tissues
2. Detection of infections caused by E. coli
3. Detection of bacterial contamination in food and water samples

Component：1. X-gluc
                       2. X-gluc Dissolved liquid
                       3. GUS Dye buffer

Storage：  Store at 4°C 

Shelf Life：2 year.