The compound 5-bromo4-chloro-3-indolyl β-D-glucuronide (X-Gluc) is a GUS substrate, which upon hydrolysis produces an indigoblue precipitate. It is used in histochemical staining of plant tissues expressing the E. coli GUS enzyme. X-Gluc is applied to tissue sections in phosphate buffer, pH 7.0, containing potassium ferricyanide and potassium ferrocyanide. The substrate penetrates the tissue and is hydrolyzed by the β-glucuronidase. The indolyl moiety obtained is then oxidized to an indoxyl radical in a reaction catalyzed by the ferricyanide/ferrocyanide mixture. The indoxyl moiety dimerizes to form an insoluble indigo-blue derivative.
1. Detection of GUS expression in plant cells and tissues
2. Detection of infections caused by E. coli
3. Detection of bacterial contamination in food and water samples
2. X-gluc Dissolved liquid
3. GUS Dye buffer
Storage： The prepared X-gluc solution store at -20°C avoid light for 20-30 days, and store at -80°C for a long time; GUS Dye buffer store at 4°C; GUS dyeing working solution was prepared when using and can store at -20°C for 2-3 days.
Shelf Life：1 year.