BCIP/NBT Solution, premixed are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble blue-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes.

Features:

1. AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
2. Chromogenic—no special equipment needed for visualization; produces purple-blue precipitate that is easy to photograph

Component：N/A

Storage： 2-8℃

Shelf Life：Store at 2-8℃ for 1 year.