Propidium iodide (or PI) is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. Therefore PI is used widely as a DNA dye in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. However, PI also binds to RNA, and it interference the DNA measurement in assay. In this Cell Cycle / Apoptosis Analysis Kit we provide a RNase to degrade RNA before PI staining to eliminate the interference by RNA and the kit can be used to monitor cell cycle progression, proliferation and apoptosis by flow cytometry.

Features:

Cell Cycle / Apoptosis Analysis Kit can be used to measure Cell Cycle / Apoptosis activity in cells

Component：1. RNase A(50×)   0.5ml
                      2. Staining Buffer   25ml
                      3. Propidium Iodide (PI)(20×) 1.25ml

Storage： -20℃. Protect from light.

Shelf Life：Store at -20℃ for 2 year.