T7 tag Mouse mAb

T7 tag（标签）单克隆抗体 

产品详情：

宿主/亚型：Mouse / IgG

靶标蛋白：T7 tag

克隆类型: Monoclonal

种属反应性：Species independent

应用: WB=1:1000-5000

纯化方式: affinity purified by Protein G

别名：T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag.  

背景介绍：

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
 The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems.

储存方式：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

1. Quick spin before use. If you have any questions, please contact us.
2. Optimal concentrations/dilutions should be determined by the end user.
3. This product is for R&D only. Not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

验证数据展示：

Primary: T7 tag Mouse mAb (BOAM-00021) at 1/1000 dilution；

Secondary: Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD Observed band size: 45 kD