Strep-Tag II Mouse mAb
Strep-Tag II(标签)单克隆抗体
产品详情:
宿主/亚型:Mouse / IgG
靶标蛋白:Strep-Tag II
克隆类型: Monoclonal
种属反应性:Species independent
应用: WB=1:1000-5000
纯化方式: affinity purified by Protein G
别名:Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG.
背景介绍:
The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
储存方式:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项:
- Quick spin before use. If you have any questions, please contact us.
- Optimal concentrations/dilutions should be determined by the end user.
- This product is for R&D only. Not for drug, household, or other uses.
- For your safety and health, please wear a lab coat and disposable gloves during the operation.
- The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
验证数据展示:
Primary: Strep-Tag II Mouse mAb(BOAM-00019) at 1/1000 dilution;
Secondary: Goat Anti-Mouse IgG at 1/20000 dilution;
Predicted band size: 51 kD Observed band size: 51 kD

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