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BOAR-00018-B Strep-Tag II Rabbit Polyclonal 标签抗体
BOAR-00018-B
Biosharp/白鲨
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Strep-Tag II Rabbit pAb

Strep-Tag II(标签)抗体

 

产品详情:

宿主/亚型:Rabbit / IgG

靶标蛋白:Strep-Tag II

克隆类型: Polyclonal

种属反应性:Species independent

应用: WB:1:500-2000;

纯化方式: affinity purified by Protein A

别名:Strep; Strep Tag II; Strep.Tag; Strep-Tag-II; Strep Tag-II; STREP II TAG.

背景介绍:

The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

储存方式:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

  1. Quick spin before use. If you have any questions, please contact us.
  2. Optimal concentrations/dilutions should be determined by the end user.
  3. This product is for R&D only. Not for drug, household, or other uses.
  4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
  5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

验证数据展示:

   

 

Primary: Strep-Tag II Rabbit pAb (BOAR-00018) at 1/1000 dilution;

Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution;

Predicted band size: 51 kD;Observed band size: 51 kD

 

 

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