GAPDH-Loading Control Mouse mAb

3-磷酸甘油醛脱氢酶（内参）单克隆抗体 

产品详情：

宿主/亚型：Mouse / IgG

靶标蛋白：GAPDH

克隆类型: Monoclonal

种属反应性：Human,Mouse,Rat,Pig,Hamster

应用:WB=1:5000-1:100000

纯化方法: affinity purified by Protein G

别名：38 kDa BFA-dependent ADP-ribosylation substrate; Aging-associated gene 9 protein;

背景介绍：

Loading Control
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. As well as functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of data appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age related neuronal diseases, such as Alzheimer’s and Huntington’s disease. GAPDH is expressed in all cells. It is constitutively expressed in almost all tissues at high levels. There are however some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types. GAPDH molecule is composed of four 36kDa subunits.

储存条件：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

1. Quick spin before use. If you have any questions, please contact us.
2. Optimal concentrations/dilutions should be determined by the end user.
3. This product is for R&D only. Not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

数据验证展示：

Primary: GAPDH (Loading Control) Mouse mAb (BOAM-00006) at 1/2000-1/20000 dilution；

Secondary: Goat Anti-Mouse IgG at 1/20000 dilution;

Predicted band size: 38 kD, Observed band size: 38 kD

Paraformaldehyde-fixed, paraffin embedded (human gastric); 

Primary: GAPDH (Loading Control) Mouse mAb (BOAM-00006) at 1/200 dilution;

Secondary Antibody: Biotin Goat Anti-mouse IgG.