His tag Mouse mAb

聚组氨酸抗体标签单克隆抗体 

产品详情：

宿主/亚型：Mouse / IgG

靶标蛋白：His tag

克隆类型: Monoclonal

种属反应性：Species independent

应用: WB：1:5000-50000

纯化方式: affinity purified by Protein G

别名：HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  

背景介绍：

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

储存方式：0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

1. Quick spin before use. If you have any questions, please contact us.
2. Optimal concentrations/dilutions should be determined by the end user.
3. This product is for R&D only. Not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

验证数据展示：

Primary: His tag Mouse mAb (BOAM-00013) at 1/1000 dilution；

Secondary: Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kD Observed band size: 45 kD

Primary: His tag Mouse mAb (BOAM-00013) at 1/200000 dilution;

Secondary: Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41 kDa, Observed band size: 45 kDa