Histone H3 (Nuclear Loading Control) Rabbit pAb

组蛋白H3（核内参）抗体

产品详情：

宿主/亚型：Rabbit / IgG

靶标蛋白：Histone H3

克隆类型: Polyclonal

种属反应性：Human,Mouse,Rat

应用: WB=1:5000-50000, IHC-P=1:500-2000, IHC-F=1:500-2000, Flow-Cyt=1μg/Test, IF=1:500-2000

纯化方式: affinity purified by Protein A

别名：H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E;

背景介绍：

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

储存条件：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

1. Quick spin before use. If you have any questions, please contact us.
2. Optimal concentrations/dilutions should be determined by the end user.
3. This product is for R&D only. Not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

验证数据展示：

Primary: Histone H3 (Nuclear Loading Control) Rabbit pAb(BOAR-00009) at 1/1000 dilution；

Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution；

Predicted band size: 17 kD；Observed band size: 15 kD

Paraformaldehyde-fixed, paraffin embedded (rat testis);

 Primary Antibody:Histone H3 (Nuclear Loading Control) Rabbit pAb(BOAR-00009) at 1/200 dilution;

Secondary Antibody: Biotin Goat Anti-Rabbit IgG.