Tubulin alpha (Loading Control) Rabbit pAb

微管蛋白α/Tubulin α（内参）抗体

产品详情：

宿主/亚型：Rabbit / IgG

靶标蛋白：Tubulin alpha

克隆类型: Polyclonal

种属反应性：Human,Mouse,Rat

应用: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, Flow-Cyt=1μg/Test, IF=1:100-500

纯化方式: affinity purified by Protein A

别名：Alpha tubulin 1; Alpha-tubulin 1; α-tubulin; α tubulin.

背景介绍：

Tubulin is a major cytoskeleton that has five distinct forms, designated alpha, beta, gamma, delta and epsilon tubulin. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP. Only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.

储存条件：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项:

1. Quick spin before use. If you have any questions, please contact us.
2. Optimal concentrations/dilutions should be determined by the end user.
3. This product is for R&D only. Not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves during the operation.
5. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

验证数据展示：

Primary: Tubulin-alpha (Loading Control) Rabbit pAb (BOAR-00002) at 1/1000 dilution；
Secondary: Goat Anti-Rabbit IgG at 1/20000 dilution；
Predicted band size: 50 kD；Observed band size: 52 kD

Blank control: RSC96 Cells (blue).
Primary Antibody:Tubulin alpha (Loading Control) Rabbit pAb (BOAR-00002), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ;
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.